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Responsibilities of clinical lab technician varies with the department you are assigned to, but usually it involves
GLP means Good Laboratory Practice. It is a framework or pattern under which research work are planned, performed, monitored, recorded, reported and archived.
By having a proper pipette training, always helps to minimize the risk of volume variability caused by Operators, also a small fraction of the change in pipetting can give you the wrong result.
An aliquot is the known amount of homogeneous material, used to minimize the sampling error. It is usually used when fractional part is an exact divisor of the whole.
Different techniques used for placing samples under micro-scope are:
Dry Mount: You simply put section of specimen with a cover slip over a sample
Wet Mount: Samples are placed under various liquid medium like glycerine, water, brine and water
Smear Slides: In this technique, sample is smear over the slide and on top it another slide is placed without forming bubbles
Squash Slides: In this technique, lens tissue is used over the wet mount, and it will remove excess water
Staining: Stains such as iodine, methylene blue and crystal violet is used to stain the specimen
The most common methods of sterilization practised in lab are:
Dry heat: Specimen containing bacteria is exposed to high temperature
Wet heat: Pressurised steam is used to kill microbes, for example, autoclave that is like pressure cooker that produces steam.
Filtration: Filtration is used where filters are as small as 0.2um is used
Radiation: UV has limited penetration, so it is generally safe to use although it is less effective to X-rays and gamma rays. X-rays and gamma rays are used only for special purposes only
Solvent: Solvent like ethanol and iso-propanol kills microbial cells but not the spores.
Sterilization: The thorough sterilization of all microbes present on the surgical instrument is referred as Sterilization>
Disinfection: While reducing the total number of microbes below the risk level is referred as Disinfection
In gas sterilization chemicals like ethylene oxide and mixture based on the substance are used for sterilizing substances. They are highly flammable and potentially explosive in nature; they are mixed with inert gases to neutralize their explosive nature.
Gas sterilization depends on factors like:
A clinical lab audit is done in order to maintain and operate the lab at a standard level.
The area that includes in clinical audit are:
Specimens: To check the patient register and see whether the specimen was received at the right time
Turnaround time: To check whether the specimen was tested and returned at allocated time, and if delayed how to improve it
GLP: To check whether the test methods carried out follows the standard procedures Purchasing equipment’s, reagents and other lab instruments
Laboratory reports: To check whether they are precise and clear and look for any area for improvement Storage of reagents and specimens
Safety policies and procedures: Use of dangerous substances should be audited, and every single accident in the lab should be recorded.
Laboratory Centrifuge is primarily used for testing liquids and substances for clinical trial samples. This device uses the centrifugal force to separate the liquids from the main sample or mixture.
When sample is rotated into centrifuge, it will separate the mixture according to the density. Supernatant is the upper layer found in the sample after it is run into centrifuge.
To avoid an imbalance in centrifuge it requires:
Balanced loading of the centrifuge rotor
Even number of tubes should be loaded facing each other or in the opposite direction
When odd number of tubes are loaded make sure, you make it even with adding one more tube with an equal amount of water of that of the sample tube
Blank term is used to refer the sample tube which does not contain the analyte.
Calibration curve is the relationship between the various concentration of analyte in a suitable solvent or matrix and the signal response of the instrument.
Co-chromatography is the procedure used to detect an unknown substance by comparing the chromatic comparison with a known substance.
For unambiguous identification of drug or metabolites in the sample, alternative chemical method is used also known as a second test.
Positive control is a specimen having the analyte at a concentration above a specific limit.
It is defined as a range over which a relationship exists between assay response and analyte concentration.
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